In this section
Macromolecular X-ray and Computing X-ray Facility
The facility is located at L141 of the main campus, 3333 Green Bay Road, North Chicago, IL 60064. It supports structural biology projects based on X-ray crystallography, single particle cryo electron microscopy and molecular modeling. Rosalind Franklin University is a member of SER-CAT (www2.ser.aps.anl.gov) with regular access to synchrotron beamlines 22ID and 22BM at Advanced Photon Source. CryoEM data is collected at National Facilities including S2C2 and NCCAT (https://nccat.nysbc.org/). Linux computing includes a 15TB-4GPU workstation for the cryoEM data processing and several graphics workstations for the x-ray crystallography. Multiple software suites are kept current by Structural Biology Grid (www.sbgrid.org). Modeling packages include Molecular Operating Environment (www.chemcomp.com) and SCHRODINGER (www.schrodinger.com). The facility have equipment as listed below.
The Core Investigators
INVESTIGATOR NAME |
PROJECT |
David H. T. Harrison, PhD, Director BMB secondary appointment |
Structural Enzymology, Methylglyoxal Synthase, HMG-CoA Synthase, and Hydroxyphenyl pyruvate |
Henry Symersky, PhD Facilities Manager |
Departmental projects utilizing macromolecular x-ray crystallography |
Carl Correll, PhD, BMB |
RNA Chaperones |
Marc Glucksman, PhD, BMB |
Structural neurobiology of processing enzymes |
Ronald Kaplan, PhD, BMB and Vice Dean of Research (CMS) |
Mitochondrial transport proteins |
Min Lu, PhD, BMB |
Multidrug and Toxin Extrusion (MATE) transporters |
David Mueller, PhD, BMB |
Yeast ATP synthase and cystic fibrosis transmembrane conductance regulator |
Kyoung Joon Oh, PhD,BMB |
Program cell death pathways |
Eric Walters, PhD, BMB |
HIV-1 protease/non-peptidyl inhibitors |
Dmitri Boudko, PhD, PB |
Transporters |
Maintenance Information:
- Confocal mirror maintenance
- Oxford Cryostream Trouble Shooting Guide
- Rigaku/MSC X-Stream Manual
- Rigaku/MSC R-axis IV detector Manual
- Rigaku/MSC RU-h2R maintenance
- Rigaku/MSC RU-h2R Expendable Parts List
Useful X-ray DataProcessing guides:
HKL-Manual
d*trekwalk through
RFUMS X-ray Facility Resources:
- Rigaku RU-h2R 5.4 kW X-ray generator with copper rotating anode
- Osmic confocal “green” fine focusing mirrors
- R-axis IV++ imaging plate area detector
- CCD video system for crystal mounting
- MSC X-Stream 2000 cryocooling system
- Bruker AXS SMART 6000 CCD detector with 3-circle goniometer
- Oxford cryocooling system
- TPP Labtech Mosquito crystallization robot
- Korima UV crystal detection and visualization system
- Liquid nitrogen
- Storage liquid nitrogen dewars
- Dry-shipping dewars
- Cryotools and crystal handling tools
- Six large incubators with temperature control
- Temperature-controlled room for crystal growth and manipulations
RFUMS CryoEM Facility Resources:
- Linux 15TB-4GPU workstation.
- PELCO easiGlowTM Glow Discharge system.
- EOL JEM-1230, 120kV Transmission Electron Microscope (TEM) equipped with a high-resolution Hamamatsu ORCA-HR CCD Camera (2624 x 2400 pixel) with AMT Imaging Software for negative stained images.
Building: BSB
Room: L.141
Phone: 847-578-8474