Calcium Imaging

The Calcium Imaging Research Support Laboratory provides the resources and expertise necessary to carry out state-of-the-art intracellular calcium measurements in cultured or freshly isolated single cells. To initiate a new project please make arrangements with the Director to discuss feasibility and technical approach.

Equipment and Capabilities

Spinning Disk Confocal Microscopy

The X-Light V2 spinning disk confocal microscope (CrestOptics) provides high temporal and spatial multicolor imaging. The system is equipped with stage incubators and perfusion baths, as well as a motorized stage and z-axis. The X-light V2 microscope provides fast scan rates and exceptionally low photo-bleaching and phototoxicity. The system is therefore ideally suited for live cell experiments requiring extended period, high frame rate acquisition. LDI-4 (89North) has four diode lasers (405, 470, 555, and 640 nm) provide the excitation, and dual view bean splitters are available for use with dual emission dyes and fluorescence resonance energy transfer (FRET) studies. VisiView image software is used for full control of image capture and data analysis. The system is also equipped with iXon Life 888 EMCCD camera (Oxford Instruments), which can generate super-resolution images using Super-Resolution Radial Fluctuations (SRRF).

Plate Reader

The BMG Polar Star Optima plate reader performs real-time kinetic monitoring of fluorescence, luminescence, or absorbance in cells grown in 24 well, 96 well or 384 well plates. Additional features include two programmable reagent injectors, temperature control up to 60°C, and analysis software features for endpoint, long-term and fast kinetic measurements.

Fee Schedule

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