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Microbiology Lab

Department of Microbiology and Immunology Facilities
Telephone: (847) 578-3230
Fax: (847) 578-3349


Bala Chandran, Ph.D.
Professor and Chair

Microbiology Lab

Department of Microbiology and Immunology Facilities
Telephone: (847) 578-3230
Fax: (847) 578-3349


Bala Chandran, Ph.D.
Professor and Chair
 
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Real-Time Quantitative PCR Support Facility

An ABI 7500 system from Applied Biosystems in Room 2.258 in the main basic science building is available for real time PCR assays. Real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (Q-PCR/qPCR) is used to amplify and simultaneously quantify targeted DNA/RNA molecules. It enables both detection and quantification of a specific sequence in a DNA/RNA sample. Its key feature is that the amplified DNA is quantified as it accumulates in the reaction in real time after each amplification cycle. Two common methods of quantification are: (1) SYBR green- fluorescent dye that intercalate with double-stranded DNA, and (2) Taqman - modified DNA oligonucleotide probe that hybridizes and fluoresces when amplifying a complementary DNA.

Possible applications of the technique include gene expression profiling, allelic exchange, quantification of alternatively spliced variant expression. 

Access:  To access the core facility, first contact Dr. Bala Chandran, Department of  Microbiology and Immunology.  After initial consultation/training by Dr. Sathish Sadagopan, user lab will be given a key for the room. 

Services:   PCR machine, 7500 sequence detection software, primer express software for primer design and printer are provided by the core facility. The users provide the supplies and reagents for the assays.

 

Lab Manager:
Dr. Mohanan Veettil
Phone (847) 578-7716		 Lab Director:
Dr. Bala Chandran
bala.chandran@rosalindfranklin.edu
Phone (847) 578-8822


 

Real-Time Quantitative PCR Support Facility

An ABI 7500 system from Applied Biosystems in Room 2.258 in the main basic science building is available for real time PCR assays. Real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (Q-PCR/qPCR) is used to amplify and simultaneously quantify targeted DNA/RNA molecules. It enables both detection and quantification of a specific sequence in a DNA/RNA sample. Its key feature is that the amplified DNA is quantified as it accumulates in the reaction in real time after each amplification cycle. Two common methods of quantification are: (1) SYBR green- fluorescent dye that intercalate with double-stranded DNA, and (2) Taqman - modified DNA oligonucleotide probe that hybridizes and fluoresces when amplifying a complementary DNA.

Possible applications of the technique include gene expression profiling, allelic exchange, quantification of alternatively spliced variant expression. 

Access:  To access the core facility, first contact Dr. Bala Chandran, Department of  Microbiology and Immunology.  After initial consultation/training by Dr. Sathish Sadagopan, user lab will be given a key for the room. 

Services:   PCR machine, 7500 sequence detection software, primer express software for primer design and printer are provided by the core facility. The users provide the supplies and reagents for the assays.

 

Lab Manager:
Dr. Mohanan Veettil
Phone (847) 578-7716		 Lab Director:
Dr. Bala Chandran
bala.chandran@rosalindfranklin.edu
Phone (847) 578-8822


 
 
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